HYBRID EVENT: You can participate in person at Tokyo, Japan or Virtually from your home or work.

3rd Edition of
World Aquaculture and Fisheries Conference

May 24-25, 2023 | Hybrid Event
WAC 2023

kai zhang

kai zhang, Speaker at Aquaculture Congress 2023
Zhejiang Normal University, China
Title : Effects of different C/N ratios on formation of biofloc, water quality and microflora in 2 aquaculture water of Chinemys reevesii

Abstract:

Abstract: In order to determine the feasibility and the best addition amount of biofloc technology in turtle culture, we 11 studied the formation of biofloc and its effects on water quality and microflora by regulating the C/N ratio in the culture 12 water of Chinemy sreevesii. We carried out the experiment with the addition of sucrose at 10?1, 15?1, 20?1 and the 13 control group for 40 d. The results show that the biofloc volume index of each group of biofloc became stable after 28 d, 14 and the treatment effect of ammonia nitrogen and nitrite was significant when the C/N ratio≥10?1. Among them, the 15 removal rate of ammonia nitrogen and nitrite after 40 d of the group with a C/N ratio of 15?1 were 76.7% and 64.4%, 16 respectively, which indicates that the C/N ratio of 15?1 can promote the formation of biofloc, and reduce the level of 17 ammonia nitrogen and nitrite in the water effectively. The biological flocs of the experimental group (C/N=15?1) and the 18 control group were sequenced by high-throughput sequencing, and the results show that the dominant phyla of biofloc 19 in the two water bodies were Proteobacteria, Bacteroidetes and Actinobacteria, but the proportion of each dominant 20 bacteria phylum was different. In conclusion, the addition of different C/N ratios can affect the formation of biofloc, water 21 quality and microflora of C.reevesii ponds. 15?1 is the optimal C/N ratio for the formation of biofloc, which can promote 22 the formation of biofloc and has a strong ability to regulate water quality. 23 Keywords: Chinemys reevesii; Biofloctechnology; Water quality indicators; High-throughput sequencing

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