Subhas Das

Title : Histotechnological technique for prediction of digestibility and liver function under choline chloride exposure: A role model on fish

Abstract:

A prediction experiment on digestibility and liver function test (LFT) was carried out on four teleostean fish [two Indian Major Carps: Catla catla (Catla), Labeo rohita (Rahu), and two air-breathing fish: Clarias batrachus (Magur), Anabas testudineus (Koi)] maintaining a cultured ratio of 2:5:1:1 :: Catla:Rahu:Magur:Koi for a period of 90-d under choline chloride exposure during dry and breeding seasons. Histological (H-E) observations of the stomach under choline exposure during dry (TD) and breeding (TB) seasons displayed a well-compacted mucosa with prominent mucosal folds comprising columnar epithelial cells (CECs) with prominent nucleus, submucosa made up of connective tissues and well-articulated gastric glands, thick muscularies layer and serosa with blood capillaries. Secretion of profuse mucus was evident from the epithelial cells through exocytosis in the breeding season due to stress and higher rate of digestibility. Histochemical  (PAS-AB) analysis displayed the prominent PAS sensitivity due to the presence of protein, whereas, the gastric gland, lamina propria and the CECs indicated very low reactivity towards PAS and negative to AB. Liver under choline exposure depicted well-articulated hepatic cords with hexagonal and/or polygonal hepatocytes showing deeply stained centrally placed nucleus and granular cytoplasm, well-articulated central veins, regular hepatopancreas, organized with compact acinar cells containing zymogen granules and sufficient colloidal mass. Histochemical (PAS-AB) analysis showed PAS positivity due to the presence of glycogen and/or mucopolysaccharides droplets in hepatopancreas, but without fatty as well as non-alcoholic steatohepatitis liver compared to control conditions. Therefore, it can be inferred that fish may be used as a role model for digestibility and LFT.

Keywords: Digestibility, Liver function test (LFT), Histological (H-E) observations, Histochemical reaction (PAS-AB), Stomach, Liver, Choline chloride

Audience take-away:

• The concept of using the choline like lipotropic factor can used for lipid metabolism
• The aquaculture entrepreneur can enhance the growth of fishes  by using this choline
• Moreover, by adopting the process of engulfing the choline (Fish as model of this experiment), liver and stomach can be free from any abnormalities

Biography:

Dr. Subhas Das is a fisheries professional and working in the Department of Fisheries, Government of West Bengal since last almost seventeen years. He has acquired Bachelor of Fishery Science (B.F.Sc.) from the West Bengal University of Animal and Fishery Sciences, West Bengal, India;  M.Sc. in Environmental Science from Vidyasagar University, West Bengal, and  Ph.D. in Environmental Science from the University of Burdwan, West Bengal. Presently he is a registered candidate of  Doctor of Science under the Department of Environmental Science, the University of Burdwan, West Bengal, India. His field of specialization under the broad subject area is Fisheries, Aquaculture, Environment and Ecology, while his major research interest under the thrust area is fish nutrition, fish immunology, eco-toxicology, biochemistry, histology, histochemistry, scanning-electron microscopy, immune-histochemistry, enzyme-histochemistry, haematology, metagenomics, amino acid profiling.