Title : Antibacterial effect of wedelia grass extract (wedelia chinensis) toward vibrio parahaemolyticus isolated from infected white leg shrimp (litopenaeus vannamei) of acute hepatopancreas necrosis disease.
Abstract:
This study aims to investigate the antibacterial effect of Wedelia grass (Wedelia chinensis) extract on Vibrio parahaemolyticus isolated from infected white-leg shrimp. The extracts of this herb were prepared in distilled water, ethanol 96% or methanol 99.8%, which were subsequently subjected to heat treatment and vacuum evaporation to remove the solvents. The diffusion method on agar was applied for the antimicrobial activity of Wedelia grass extract against V. parahaemolyticus. The diameters of the antibacterial zone of wedelia grass extract in the distilled water, ethanol 96% and methanol 99.8 % were 12.1mm, 11.7mm, and 14.7mm, respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) results of the wedelia grass extract in methanol 99.8% exhibited the most potent antibacterial activity against V. parahaemolyticus at 15.5 mg/mL and 31.25 mg/mL, respectively. Wedelia grass extract in methanol 99.8% was non-toxic and non-lethal when feeding shrimps at the dose of MBC (31,25 mg/mL). This study demonstrates the potential of using the extraction of wedelia grass in the prevention and treatment of V. parahaemolyticus infection in shrimp.
Keywords: White leg shrimp, Wedelia grass extract, Antimicrobiology, Inhibitory concentration, and Safety.
What will the audience learn from your presentation?
- Provide practical solution of using herbs (Wedelia grass extract) in the prevention and treatment of AHPND in white leg shrimp for the farmers to reduce the risk of this disease in shrimp farming
- Provide scientific information for other researchers to study the safe solution for the prevention and treatment of bacterial diseases in an aquatic animal to reduce the using antibiotics in aquaculture.
