HYBRID EVENT: You can participate in person at Paris, France or Virtually from your home or work.

4th Edition of
World Aquaculture and Fisheries Conference

June 24-26, 2024 | Paris, France
WAC 2023

Banikalyan Swain

Banikalyan Swain, Speaker at Aquaculture Conference
University of Florida, United States
Title : Design and construction of generalized vaccine-vector system to protect teleost fish against multiple bacterial, viral and parasitic infectious diseases in aquaculture


We have successfully designed and constructed a RAEV vector system with regulated-delayed attenuation in vivo attributes that synthesizes Ichthyophthirius multifiliis (Ich) protective antigen IAG52B to enable vaccination of fish susceptible to edwardsiellosis and white spot disease. The first feature of this vaccine delivery system is an Edwardsiella piscicida strain carrying genomic deletions of asdA.  AsdA is an enzyme necessary for the synthesis of diaminopimelic acid (DAP), which is an essential component of the peptidoglycan layer of the cell wall of Gram-negative bacteria. asdA mutant strains have obligate growth requirements for DAP in the medium or a plasmid vector with the wild-type asdA gene enabling synthesis of DAP. This balanced-lethal plasmid vector-host system in E. piscicida enables as a second feature the synthesis of recombinant antigens to induce protective immunity against fish pathogens. Recombinant protective antigen IAG52B from the fish pathogen I. multifiliis was synthesized by RAEV strains harboring the AsdA+ plasmid pG8R8029. The third feature of this vaccine strain is a regulated-delayed attenuation in vivo phenotype that is based on the replacement of an arabinose-regulated araC ParaBAD cassette for the promoters of the fur and crp genes of E. piscicida such that the expression of these genes is dependent on arabinose provided during growth. Thus, following colonization, the Fur and Crp proteins stop being synthesized due to the lack of arabinose and attenuation is progressively achieved in vivo to prevent generation of diseases symptoms. Our vaccine strain c16022 with the genotype ?asdA10 ΔPfur170::TT araC ParaBAD fur DPcrp68::TT araC ParaBAD crp contains the AsdA+ plasmid, pG8R8029, which encodes the IAG52B antigen.  Vaccine strain c16022(pG8R8029) is attenuated and induces systemic and mucosal IgM titer against E. piscicida and Ich in zebrafish. In addition, transcript levels of tnf-α, il-1β, il-6 and il-8 were significantly increased in different tissues of vaccinated zebrafish compared to unimmunized fish. Zebrafish vaccinated with c16022(pG8R8029) showed 60% survival upon intracoelomic (i.c.) challenge with a lethal dose of virulent E. piscicida strain J118.

We also constructed and synthesized Tilapia Lake Virus (TiLV) antigens (Segment 5 and 6) and Aeromonas hydrophila antigens in RAEV, with the expression vector pG8R114. Use of pG8R114 vector with the fusion of antigens to the bla SS (T2SS) leads to the delivery of antigens to the periplasm, resulting in an increased production of outer membrane vesicles (OMVs) that enhance immunogenicity and antibody production against delivered antigens. Zebrafish immunized with RAEV harboring pG8R114-TonB showed 60% higher survival compared to the control groups against virulent A. hydrophila challenge. We developed a new mucosal antigen delivery RAEV system for aquaculture that will prevent important infectious diseases and therefore increase the sustainability and profitability of the finfish aquaculture industry.


Dr. Banikalyan Swain obtained his Ph.D. in Biotechnology from Utkal University in 2015, Bhubaneswar, India. He joined University of Florida as a Postdoctoral Associate in 2015. Currently he is working as a Research Assistant Professor in Department of Infectious Diseases and Immunology, University of Florida. Dr. Swain focused on innate and adaptive immune mechanism of fish and he has published his research in different reputed peer reviewed journals. He is working on the design, construction and evaluation of genetically modified derivatives of Edwardsiella piscicida, as vaccine vectors to deliver protective antigens encoded by genes from other pathogens as well as DNA vaccines encoding such protective antigens. Dr. Swain and his group has designed a regulated delayed attenuation and programmed self-destructing Edwardsiella piscicida strains that efficiently colonize fish lymphoid tissues, allow release of the bacterial cell contents after lysis and exhibit complete biological containment. These technologies are being used to reduce morbidity and mortality caused by bacterial, viral and parasite infectious disease agents of fish.