Title : Efficacy of quorum quenching on the prevention of acute hepatopancreatic necrosis disease(AHPND) in the white leg shrimp
Abstract:
Aquaculture is an important food-producing sector to fulfill the nutritional food demand of a continuously growing population, accounting for 46 percent of the total production and 52 percent of fish for human consumption (FAO, 2020). However, disease outbreaks especially Acute hepato-pancreatic Necrosis Diseases (AHPND) caused by Vibrio parahaemolyticus is now considered to be the limiting factor in the shrimp culture subsector. Due to the widespread resistance and the current poor effect of antibiotics consumed to treat V. parahaemolyticus infections, finding some novel alternative therapeutic methods is necessary for the treatment of infections. Bacterial communication through signaling molecules known as “quorum sensing” (QS) has gained a lot of attention due to its applicability in different fields of study. Quorum sensing inhibitors (QSIs) or quorum quenchings (QQs) are now considered as potential therapeutic alternatives and/or adjuvants to the current failing antibiotics. The aims of this study were to assess the efficacy of QQ products on the prevention of AHPND in white leg shrimp. In this study, the effect of QQ on the outcome of the experimental challenge of white leg shrimp with V. parahaemolyticus, shrimp were held at a stocking density of 30 shrimp per tank for 2 days before the challenge. Challenges were conducted with 8 treatments in triplicate (three tanks per treatment) at day 2 by immersion with 10L of the LD50 for 30 min. Shrimp were fed with a commercial diet (N1, CP, Vietnam) with or without the addition of (0.5 or 1%) QQ for 8 days. This study showed that QQ increased the THC, phagocyte, phenoloxidase, and Lysozyme activity in shrimp. The addition of 0.5 or 1% QQ in the feed can reduce the mortality of white shrimp when infected with V. parahaemolyticus. These results showed that QQ (in both 0.5 or 1% QQ addition) was beneficial for white shrimp in terms of resisting challenge by Vibrio paraheamolyticus and enhancing cellular and humoral immune responses
What will audience learn from your presentation?
Due to the widespread resistance and the current poor effect of antibiotics consumed to treat V. parahaemolyticus infections, this study provides the novel alternative therapeutic method which is necessary for the treatment of infections. Besides, this study provides the scientific information about the immune response and disease prevention of shrimp to the researchers and practical methods to the farmer in the bacterial disease prevention.
