Shixi Chen

Title : Sequence analysis of nuclear ITS1-5.8S-ITS2 of white Channa argus

Abstract:

Channa argus is widely distributed and extremely resistant to hypoxia, it  is now an aquaculture species in China, and has heritable white body  color populations with two phenotypes of "gray fin rays" and "gold fin rays". In this study, the genetic diversity and molecular phylogenetics of  C. argus with different body colors and geographical distribution are investigated based on their nuclear ITS1-5.8S-ITS2 sequences. The ITS1-5.8S-ITS2 sequences of the C. argus are conservative, with 1/1000 of mutation sites and 0.000-0.014 genetic distance indicates the C. argus that was studied has the same ITS1-5.8S-ITS2 sequences and genetic distance, all of which show that it is the same species. The intraspecific variation sites shows that it was mainly transformation, white C. argus with "gray fin rays" individuals have more variation sites than "gold fin rays" individuals. and the variation associated with different geographical distributions is smaller than that associated with different body colors. Therefore, these sequences can be used to identify the differences between C. argus in different areas, which shows that nuclear ITS1-5.8S-ITS2 sequences can be used to study molecular phylogeography. Channa argus is widely distributed and extremely resistant to hypoxia, it  is now an aquaculture species in China, and has heritable white body  color populations with two phenotypes of "gray fin rays" and "gold fin rays". In this study, the genetic diversity and molecular phylogenetics of  C. argus with different body colors and geographical distribution are investigated based on their nuclear ITS1-5.8S-ITS2 sequences. The ITS1-5.8S-ITS2 sequences of the C. argus are conservative, with 1/1000 of mutation sites and 0.000-0.014 genetic distance indicates the C. argus that was studied has the same ITS1-5.8S-ITS2 sequences and genetic distance, all of which show that it is the same species. The intraspecific variation sites shows that it was mainly transformation, white C. argus with "gray fin rays" individuals have more variation sites than "gold fin rays" individuals. and the variation associated with different geographical distributions is smaller than that associated with different body colors. Therefore, these sequences can be used to identify the differences between C. argus in different areas, which shows that nuclear ITS1-5.8S-ITS2 sequences can be used to study molecular phylogeography. 

Biography:

Dear academics and professors, as a lecturer at Neijiang Normal University in Neijiang, Sichuan Province, 641100, China, Dr. Shixi Chen researched about fish hypoxic stress, fish genetics, particularly the recent discovery of two types of albino body color populations in Channa argus (snakeheads), and the mitochondrial genomes of gigantic clams (Tridacnidae spp.), chloroplast genome plants (Thalictrum fargesii) are also involved in his study. If interested in his research.